Human Papillomavirus Type 16 E6* Induces Oxidative Stress and DNA Damage
نویسندگان
چکیده
منابع مشابه
Human papillomavirus type 16 E2 and E6/E7 variants.
OBJECTIVES Polymorphisms in human papillomavirus (HPV) type 16 have been shown to be related to geographic areas and are broadly classified as European (E), African (Af), Asian (As), or Asian-American (AA). Certain variants have been reported as being more likely to cause cervical disease; our objectives were to identify new HPV16 polymorphisms, to determine the linkage of the E2 and E6/E7 regi...
متن کاملHuman papillomavirus type 16 E6 induces self-ubiquitination of the E6AP ubiquitin-protein ligase.
The E6 protein of the high-risk human papillomaviruses (HPVs) and the cellular ubiquitin-protein ligase E6AP form a complex which causes the ubiquitination and degradation of p53. We show here that HPV16 E6 promotes the ubiquitination and degradation of E6AP itself. The half-life of E6AP is shorter in HPV-positive cervical cancer cells than in HPV-negative cervical cancer cells, and E6AP is sta...
متن کاملDevelopment of a DNA vaccine targeting human papillomavirus type 16 oncoprotein E6.
Human papillomavirus (HPV), particularly type 16 (HPV-16), is present in more than 99% of cervical cancers. The HPV oncoproteins E6 and E7 are constantly expressed and therefore represent ideal targets for HPV vaccine development. We previously developed DNA vaccines encoding calreticulin (CRT) linked to HPV-16 E7 and generated potent E7-specific CD8(+) T-cell immune responses and antitumor eff...
متن کاملDelayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.
A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By...
متن کاملPleiotropic effects of human papillomavirus type 16 E6 oncogene expression in human epithelial cell lines.
Two human papillomavirus (HPV)-negative epithelial cell lines, HaCaT and C33A, were transfected with HPV-16 E6 and analysed for functional consequences which are relevant to invasive tumour progression. After transfection with E6, both cell lines invaded collagen matrices, in contrast to vector-transfected control cells. The E6-expressing cells showed a marked increase in expression of the beta...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2014
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.03355-13